Journal of Applied Sciences and Environmental Management
World Bank assisted National Agricultural Research Project (NARP) - University of Port Harcourt
Vol. 21, No. 6, 2017, pp. 1143-1147
Bioline Code: ja17140
Full paper language: English
Document type: Research Article
Document available free of charge
Journal of Applied Sciences and Environmental Management, Vol. 21, No. 6, 2017, pp. 1143-1147
© Copyright 2017 - Journal of Applied Sciences and Environmental Management
Assessment of Genetic Variability in Sorghum Accessions ( Sorghum bicolor L. Moench) at the National Centre for Genetic Resources and Biotechnology, Ibadan, Nigeria|
SIFAU, MO; ODUOYE, OT; OLUWASANYA, OA & ALADELE, SE
The determination of genetic variation using molecular markers has been found to facilitate the conservation of crops and
ensure food security. Genetic diversity among 80 accessions of S. bicolor in the gene bank of National Centre for Genetic
Resources and Biotechnology (NACGRAB) Ibadan, was studied using 5 pairs of simple sequence repeat (SSR) markers.
The polymorphic information content (PIC) of individual primer ranged from 0.34 to 0.70 with a mean value of 0.54
indicating enough diversity or variability among the accessions studied. The binary matrix obtained from the gel profiles
generated a dendrogram which was made up of 4 clusters and one ungrouped accession at 0.66 coefficients of similarity.
From the clustering pattern, 7 pairs of accessions were found to be 100% similar. Each similar pairs were subsequently
merged together and reduced to a total of 7 accessions. However, it was also observed that the geographical location of
collection of accessions did not affect the clustering pattern. The information obtained from this study could serve as the
basis for the improvement and breeding programs of Sorghum to achieve food security in the country, and by extension,
Sorghum; Simple Sequence Repeat markers; Genetic variation; Polymorphic Information Content; Coefficient of similarity.