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Journal of Applied Sciences and Environmental Management
World Bank assisted National Agricultural Research Project (NARP) - University of Port Harcourt
ISSN: 1119-8362
Vol. 21, No. 6, 2017, pp. 1169-1173
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Bioline Code: ja17145
Full paper language: English
Document type: Research Article
Document available free of charge
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Journal of Applied Sciences and Environmental Management, Vol. 21, No. 6, 2017, pp. 1169-1173
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Evaluation on the growth response of Peanut ( Arachis hypogaea ) and Sorghum ( Sorghum bicolor ) to crude oil contaminated soil
IHEME, P O; AKINOLA, M O & NJOKU, K L
Abstract
This study examined the cytotoxic response, germination, survival, morphological deviations as well
as enzyme activities of Arachis hypogaea and Sorghum bicolor in crude oil contaminated soil. Crude oil spillage was
simulated to achieve 1%, 2%, 3% (w/w) contamination levels in pot experiments. Treatments without crude oil were
used as control. Cytotoxicity, germination and survival were determined by using percentages while enzyme activity
was measured by using spectrophotometric methods and standard curves. S. bicolor had lower mitotic index (3.7) with
higher percentage aberrations (65.56%) compared to A. hypogaea. However, difference in mitotic index and percentage
aberration between A. hypogaea and S. bicolor was not significant at P≥0.05. Percentage germination and survival of
both plants were not different. Enzyme study showed that enzyme activity in A. hypogaea and S bicolor were the same
in control but increased with crude oil contamination. Laccase activity was significantly higher (P≤0.05) in A. hypogaea
than in S. bicolor in 1% and 2% crude oil concentrations. The same applies to soluble methane monoxygenase activity
in all crude oil concentrations. Tyrosinase activity was not significantly different in both plants in all concentrations.
The study shows A. hypogaea to have better tolerance in crude oil contaminated soils than S. bicolor.
Keywords
S. bicolor; A. hypogaea; crude oil; pollution; response
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© Copyright 2017 - Journal of Applied Sciences and Environmental Management
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