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Journal of Applied Sciences and Environmental Management
World Bank assisted National Agricultural Research Project (NARP) - University of Port Harcourt
ISSN: 1119-8362
Vol. 22, No. 1, 2018, pp. 54-59
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Bioline Code: ja18010
Full paper language: English
Document type: Research Article
Document available free of charge
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Journal of Applied Sciences and Environmental Management, Vol. 22, No. 1, 2018, pp. 54-59
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Optimization of bioethanol production from simultaneous saccharification and fermentation of pineapple peels using Saccharomyces cerevisiae 
OIWOH, O; AYODELE, BV; AMENAGHAWON, NA & OKIEIMEN, CO
Abstract
In this study, bioethanol production from the simultaneous saccharification and fermentation (SSF) of
pineapple peels using cellulase and Saccharomyces cerevisiae was investigated. A three-factor Box-behnken design
(BBD) and response surface methodology (RSM) were employed to study the effect of broth pH (2-6), yeast loading (2-10 g/l) and ammonium sulphate concentration (1-5 g/l) on the bioethanol production process. Optimum values of pH,
yeast loading and ammonium sulphate concentration of 6.0, 8ml and 5g/l, respectively were obtained for maximum
bioethanol concentration of 5.82%v/v. The results obtained show the possibility of using pineapple peels as feedstock
for bioethanol production via SSF method. Moreover, the use of BBD and RSM as robust technique for determining the
effect of parameters and optimum conditions for bioethanol production has been ascertained.
Keywords
Bioethanol; Box-behnken design; Fermentation; Optimization; Pineapple peels
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© Copyright 2017 - Oiwoh et al.
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