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Journal of Applied Sciences and Environmental Management
World Bank assisted National Agricultural Research Project (NARP) - University of Port Harcourt
ISSN: 1119-8362
Vol. 22, No. 3, 2018, pp. 304-307
Bioline Code: ja18053
Full paper language: English
Document type: Research Article
Document available free of charge

Journal of Applied Sciences and Environmental Management, Vol. 22, No. 3, 2018, pp. 304-307

 en Effects of Pesticide Application on the Growth of Soil Nitrifying Bacteria


This research studied the effects of pesticide application on the growth of soil nitrifying bacteria. Nitrosomonas check for this species in other resources sp. was isolated from the Soil samples gotten from five different locations within the University of Benin, Benin City and cultured using Winogradsky medium. The bacterial isolate was characterized and identified based on its cultural, morphological and biochemical characteristics. After 4 to 5 days of growth, Nitrosomonas Counts obtained from Winogradsky medium ranged from 4.8×103 to 9.2×103cfu/g. There was an increase in bacteria growth at lower pesticide concentration which began to decrease as the pesticide concentration increased with time. The percentage nitrite accumulation gradually decreased with time, the LC50 values were higher than EC50 values which shows that the bacteria could survive and grow at lower pesticide concentrations but were completely inhibited at higher pesticide concentrations. It is therefore very important to examine pesticides so as to determine its toxicity to soil bacteria before application.

Pesticide; nitrosomonas sp.; winogradsky medium; nitrite accumulation

© Copyright 2018 - Udochukwu et al.

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