search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Journal of Applied Sciences and Environmental Management
World Bank assisted National Agricultural Research Project (NARP) - University of Port Harcourt
ISSN: 1119-8362
Vol. 22, No. 4, 2018, pp. 565-570
Bioline Code: ja18099
Full paper language: English
Document type: Research Article
Document available free of charge

Journal of Applied Sciences and Environmental Management, Vol. 22, No. 4, 2018, pp. 565-570

 en Management of Seed-Borne Fungi in Cowpea Using Leaf Extracts and Sodium Bicarbonate
KAREEM, KT; AFOLABI, QO; SHORINMADE, AY & AKINBODE, OA

Abstract

The study investigated the in-vitro antifungal activities of sodium bicarbonate (NaHCO3) and leaf extracts of Vernonia amydgalina check for this species in other resources (bitter leaf) and Azadirachta indica check for this species in other resources (neem) on fungal species isolated from two cowpea cultivars; IFE BROWN and Drum (a local cultivar). Concentrations of 1.95 mg/ml and 1.43 mg/ml were used for A. indica while 1.45 mg/ml and 1.15 mg/ml were used for V. amydgalina. Sodium bicarbonate was evaluated on all the isolates at 2.0 mg/ml and 3.0 mg/ml. The inhibitory effects of these antifungal agents were compared with benlate (a synthetic fungicide) on Potato Dextrose Agar. Benlate totally inhibited all the fungal isolates. Candida check for this species in other resources species isolated from the two cowpea cultivars were greatly inhibited by the antifungal agents while Trichoderma check for this species in other resources species isolated from IFE BROWN was the least inhibited. The inhibitory effects of NaHCO3 and A. indica on Candida spp. were not significantly different and values ranged from 74.5% to 84%. The two concentrations of A. indica had better inhibitory effect on Pythium check for this species in other resources spp. than NaHCO3. Vernonia amydgalina at 1.45 mg/ml inhibited A. niger check for this species in other resources and Candida spp. by 83.75% and 87.5% respectively while NaHCO3 at 2.0 mg/ml inhibited Rhizopus stolonifer check for this species in other resources isolated from Drum by 75%. The study concludes that the two plant extracts and sodium bicarbonate had broad spectrum activities on fungal species and are therefore recommended as alternatives to toxic and synthetic fungicides.

Keywords
Cowpea; eco-friendly; fungicide; inhibition

 
© Copyright 2018 - Kareem et al.

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2019, Site last up-dated on 11-Sep-2019.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil