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Journal of Applied Sciences and Environmental Management
World Bank assisted National Agricultural Research Project (NARP) - University of Port Harcourt
ISSN: 1119-8362
Vol. 22, No. 5, 2018, pp. 613-618
Bioline Code: ja18107
Full paper language: English
Document type: Research Article
Document available free of charge

Journal of Applied Sciences and Environmental Management, Vol. 22, No. 5, 2018, pp. 613-618

 en Extraction and Fractionation of Whole Black Seed Plantain ( Plantago rugelii check for this species in other resources Decne) for in-vitro Antioxidant, Antibacterial and Phytochemical Screening
OGBIKO, C; EBOKA, CJ & TAMBUWAL, AD

Abstract

This study was designed to explore new antioxidant and antimicrobial agents from the methanol whole plant crude extract and fractions of Plantago rugelii. The methanol extract and its fractions were prepared and screened for its phytochemical composition, in-vitro antioxidant potential and challenged with common pathogenic microorganisms for its antimicrobial activities using standard procedures. The phytochemical analysis revealed the presence of various pharmaceutically active secondary metabolites like alkaloids, phenolic, flavonoids, carbohydrates, glycosides, sterols, etc. In the DPPH assay, the aqueous methanol fraction was found to be the most effective among all the fractions in comparison to the ascorbic acid standard. Using the zone of inhibition as inhibitory parameter, the crude methanol extract exhibited the best antibacterial activity when challenged against all the clinical isolates except Klebsiella pneumoniae check for this species in other resources . The aqueous methanol extract exhibited the best percentage fungal inhibition when compared to other fractions. The standard drugs ciprofloxacin and fluconazole exhibited a near 100% activity except with Proteus vulgaris check for this species in other resources where the crude methanol extract has a higher value.This research holds promise for the exploration of various potentially active secondary metabolites which would help in developing pharmaceuticals, especially antioxidant and antimicrobial drugs. The isolation and characterization of the exact metabolites responsible for these activities is therefore recommended.

Keywords
Plantago rugelii; Antioxidant; Free Radicals; Antimicrobial

 
© Copyright 2018 - Ogbiko et al.

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