Journal of Applied Sciences and Environmental Management
World Bank assisted National Agricultural Research Project (NARP) - University of Port Harcourt
Vol. 22, No. 7, 2018, pp. 1065-1070
Bioline Code: ja18182
Full paper language: English
Document type: Research Article
Document available free of charge
Journal of Applied Sciences and Environmental Management, Vol. 22, No. 7, 2018, pp. 1065-1070
© Copyright 2018 - Aina et al.
Biodegradation of Naphthalene using Pseudomonas putida and Bacillus subtilis Immobilized on Snail Shell|
AINA, OE; AGBAJI, EB & NWOKEM, NC
This study investigated snail shell as a carrier to immobilize Pseudomonas putida and Bacillus subtilis
isolated from refinery effluent for the degradation of naphthalene in synthetic wastewater at various process conditions
such as initial naphthalene concentration, pH, adsorbent dosage and ambient temperature of 30 oC in batch mode. The
results showed that the adsorption and the biodegradation capacity increased with increase in naphthalene concentration,
where 73.11%, 74.46% and 65.20% of the optimum concentration (50 mg/L) were removed by immobilized Pseudomonas
putida, Bacillus subtilis, and snail shell respectively after 72 hours incubation. The optimal degradation occurred at the
adsorbent dosage of 2 g at pH 9 and pH 7 for the adsorption and biodegradation respectively. The results were well fitted
to both Langmuir and Freundlich models. Therefore, snail shell can be employed as a low-cost adsorbent and solid support
matrix for immobilizing microorganisms in remediating hydrocarbon contaminants.
Biodegradation; Naphthalene; Immobilization; Snail shell.