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Journal of Applied Sciences and Environmental Management
World Bank assisted National Agricultural Research Project (NARP) - University of Port Harcourt
ISSN: 1119-8362
Vol. 22, No. 10, 2018, pp. 1595-1598
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Bioline Code: ja18267
Full paper language: English
Document type: Research Article
Document available free of charge
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Journal of Applied Sciences and Environmental Management, Vol. 22, No. 10, 2018, pp. 1595-1598
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Evaluation of Optimum Concentration of Naphthalene Acetic Acid on in Vitro Rooting and Acclimatization of Tissue Culture Date Palm ( Phoenix dactylifera L.) Plantlets
EMOGHENE, BO; ASEMOTA, O; EKE, CR; IDU, M; AGHIMIEN, E & NWITE, AP
Abstract
A major limitation in large scale application of micropropagation technology is high mortality
experienced by in vitro raised plants during laboratory to land transfer. This study was aimed at investigating the best
concentration of Naphthalene acetic acid (NAA) on in vitro rooting and also develop protocol for successful plant
acclimatization with high survival rate. Date palm plantlets were rooted on Murashige and Skoog (MS) basal medium
with different NAA concentrations (0.0, 0.1, 0.5 1.0, 1.5 and 2 mg/l) without activated charcoal. Result showed that all
the NAA concentrations supported root formation. The optimal initiation and growth of roots of the plantlets were
observed at NAA concentration of 1.0 mg/l (87 %) and least with Basal MS without NAA (47 %). Results also showed
that root number and lengths increased with increasing NAA concentrations up to 1.0 mg/l and decreased thereafter. The
culture mixture of peat moss + soil (2:1) gave the highest survival percentage of 80 % for plant acclimatization. This
investigation had shown that accurate assessments of responses to medium at various stages should be considered as
specific requirement for successful plant acclimatization.
Keywords
date palm; in vitro; plantlets; acclimatization
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© Copyright 2018 - Emoghene et al.
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