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Isolation and Characterization of Β-Sitosterol from Methanol Extracts of the Stem Bark of Large- Leaved Rock Fig ( Ficus Abutilifolia  Miq)
ALIBA, MO; NDUKWE, IG & IBRAHIM, H
Abstract
This work was aimed at isolating and characterizing some bioactive constituent(s) from the stem
bark of Ficus abutilifolia. The phytochemical analysis of the crude methanol extract indicates the presence of
flavonoids, alkaloids, cardiac glycosides, saponnins, tannin, steroids and triterpenes. The extract was subjected to
column chromatography which resulted in the isolation and purification of a white needle-like crystalline substance
with a melting point of 134 – 1360C, the 13CNMR shows recognizable peak at 71.98 ppm assignable to the beta hydroxyl
group and the 1HNMR spectrum which shows an olefinic proton at δ 5.37 was identified as β-sitosterol. Identification
of the compound was purely by spectral evidence.
Keywords
Ficus abutilifolia; methanol extracts; phytochemical analysis; β-sitosterol.
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