search
for
 About Bioline  All Journals  Testimonials  Membership  News


Journal of Applied Sciences and Environmental Management
World Bank assisted National Agricultural Research Project (NARP) - University of Port Harcourt
ISSN: 1119-8362
Vol. 24, No. 12, 2020, pp. 2161-2165
Bioline Code: ja20309
Full paper language: English
Document type: Research Article
Document available free of charge

Journal of Applied Sciences and Environmental Management, Vol. 24, No. 12, 2020, pp. 2161-2165

 en Genetic Variability and Vegetative Compatibility in Aspergillus niger check for this species in other resources Isolated from Various Food Substances in Benin City Nigeria
Eboigbe, L & Omoregbe, MO

Abstract

In this investigation, Aspergillus niger isolated from eight food substances, have been classified based on the absence of heterokaryon formation. The size of their sporangia were differentiated, the wild and mutant strains were subjected to vegetative compatibility tests in order to group them into different vegetative compatibility groups (VCGs) which include VCG-1, VCG-2, VCG-3 and VCG-4. The strains were further tested for the possible formation of a stable heterokaryon using nit mutants generated on potato dextrose agar (PDA) containing 2.5% chlorate (KClO3), represented as PDC. Based on the vegetative compatibility groups, nit mutants were paired on a minimal medium (MM) for complementation test. Interestingly, there was compatibility with mycelia showing anastomoses but without the formation of heterokaryon. The vegetative compatibility groups suggested four genotypes and polymorphism in the het loci. A population study for detailed genotyping is suggested in order to unravel the genetic recombination in A. niger.

Keywords
Vegetative compatibility; heterokaryon; Het loci; genotype; mutant; polymorphism

 
© Copyright 2020 - Eboigbe and Omoregbe

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2025, Site last up-dated on 01-Sep-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Google Cloud Platform, GCP, Brazil