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African Journal of Health Sciences
The Kenya Medical Research Institute (KEMRI)
ISSN: 1022-9272
Vol. 13, No. 3-4, 2006, pp. 66-69
Bioline Code: jh06030
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Health Sciences, Vol. 13, No. 3-4, 2006, pp. 66-69

 en Tissue Microarrays and Their use for Preparation of Reference Slides for Educational Purposes in Histology and Histopathology
Deeb, Salah; Badii, Mohamed; El-Begawey, Mahmoud; Ali, Khalid & Mahdi, Emad

Abstract

Use of Tissue array was first applied in 1998, and has received a significant amount of attention from the research community ever since. In this technique, a large number (up to 1000) of cylindrical tissue core extracted from "donor" paraffin block are deposited into "recipient" block. The aim was modification of the technique of tissue array for manual preparation of the recipient block and production of slides of educational interest in histology and histopathology. The area of interest was localized with the help of stained section, the area was punctured, and the cylindrical core of tissue removed was then introduced into another (recipient) paraffin block. Puncture method was suitable only for parenchymatous organs (liver, kidney, heart, spleen, etc.) but longitudinal sections were required for tubular (gastrointestinal tract, urinary tract, genital tract) and hollow organs (gallbladder, urinary bladder) and brain. The method described is of importance in procurement of materials for preparation of slides for educational purposes and in overcoming the shortage of these materials especially in the field of pathology

 
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