Reference Physiological Ranges for Serum Biochemical Parameters among Healthy Cameroonians to Support HIV Vaccine and Related Clinical Trials|
Alemnji, G. A.; Mbuagbaw, J.; Folefac, E.; Teto, G.; Nkengafac, S.; Atems, N.; Kwingwah, B. B.W. & Asonganyi, T
Background: A valid scientiﬁc evaluation of the eﬃcacy of HIV vaccines or antiretroviral
drugs includes measurement of changes in physiological parameters of subjects from known
established baseline reference ranges. This study was designed to establish reference ranges for
biochemical parameters among healthy adult Cameroonians to support planned HIV Vaccine
clinical trials and scaling up of ARV drugs among AIDS patients.
Methods: After informed consent, blood and urine samples were collected from a total of 576
adult Cameroonians and analyzed for the presence of underlying pathologies that may aﬀect
biochemical parameters. Samples from 501 of them were found eligible for the determination
of reference biochemical parameters. After complete assay, the data were subjected to both
parametric and non parametric statistics for analyses with 2.5 and 97.5 percentiles considered
as the lower and upper limits of reference ranges.
Results: There were 331 (66.1%) males and 170 (33.9) females, with 359 (71.7%) and 142 (28.3%)
of them residing in the urban and rural areas respectively. Statistically signiﬁcant diﬀerences
(P<0.05) were observed in the following biochemical parameters between urban and rural
participants: AST, ALT, alkaline phosphatase, creatinine, total protein, albumin, triglyceride,
total cholesterol, and the bilirubins. When the data were regrouped into sex, there were
statistically signiﬁcant diﬀerences (P<0.05) in the following parameters between males and
females: AST, ALT, creatinine, albumin, triglyceride, total cholesterol, and direct bilirubin.
Conclusion: The present study shows that sex and geographic location have signiﬁcant impact
on reference physiological biochemical parameters of healthy, adult Cameroonians; hence this
should be taken into consideration when monitoring participants either during HIV Vaccine
clinical trials or on antiretroviral (ARV) drugs treatment.
Normal Biochemical Ranges; Healthy Adult Cameroonians