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Indian Journal of Medical Microbiology
Medknow Publications on behalf of Indian Association of Medical Microbiology
ISSN: 0255-0857 EISSN: 0255-0857
Vol. 25, No. 2, 2007, pp. 140-142
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Bioline Code: mb07039
Full paper language: English
Document type: Research Article
Document available free of charge
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Indian Journal of Medical Microbiology, Vol. 25, No. 2, 2007, pp. 140-142
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Brief Communication - Evaluation of commercially available third-generation anti-hepatitis C virus enzyme-linked immunosorbent assay in patients on haemodialysis
Lakshmi, V; Reddy, AK & Dakshinamurty, KV
Abstract
Restricted antibody reactivity to hepatitis C virus (HCV) synthetic peptides has been observed in HCV-infected patients on haemodialysis (HD). The aim of this study was to evaluate third-generation anti-HCV enzyme-linked immunosorbent assay (ELISA) test systems containing either synthetic peptide HCV antigens or recombinant HCV antigens or a combination of synthetic and recombinant antigens in screening of 69 chronic renal failure patients on HD for HCV infection. Seven patients were detected to have antibodies to HCV by the 'recombinant HCV antigens'-containing kits, of which the recombinant immunoblot assay for HCV confirmed four cases. The recombinant kits had a sensitivity of 100% and a specificity of 66%. However, the ELISA kits with only synthetic HCV antigens failed to detect antibodies in any of the cases (zero sensitivity). Hence a recombinant protein containing ELISA test system is ideal for screening of HCV infection in patients on hemodialysis.
Keywords
Chronic renal failure, enzyme-linked immunosorbent assay, hepatitis C virus, haemodialysis, synthetic peptides
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© Copyright 2007 Indian Journal of Medical Microbiology. Alternative site location: http://www.ijmm.org
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