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Indian Journal of Medical Microbiology
Medknow Publications on behalf of Indian Association of Medical Microbiology
ISSN: 0255-0857
EISSN: 1998-3646
Vol. 26, No. 4, 2008, pp. 322-326
Bioline Code: mb08102
Full paper language: English
Document type: Research Article
Document available free of charge

Indian Journal of Medical Microbiology, Vol. 26, No. 4, 2008, pp. 322-326

 en Antiviral activity of the Indian medicinal plant extract, Swertia chirata check for this species in other resources against herpes simplex viruses: A study by in-vitro and molecular approach
Verma, H; Patil, PR; Kolhapure, RM & Gopalkrishna, V

Abstract

Purpose: The antiviral activity of Indian Medicinal plant extract Swertia chirata check for this species in other resources was tested against Herpes simplex virus (HSV) type-1, using multiple approaches both at cellular and molecular level.
Methods: Cytotoxicity, plaque reduction, virus infectivity, antigen expression and polymerase chain reaction (PCR) assays were conducted to test the antiviral activity of the plant extract.
Results: Swertia plant crude extract (1gm/mL) at 1:64 dilution inhibited HSV-1, plaque formation at more than 70% level. HSV antigen expression and time kinetics experiments conducted by indirect immunofluorescence (IFA) test, revealed a characteristic pattern of small foci of single fluorescent cells in Swertia extract treated HSV-1 infected cells at 4 hours post infection dose, suggested drug inhibited viral dissemination. Infected cell cultures treated with Swertia extract at various time intervals, tested by PCR, failed to show amplification at 12, 24-72 hours. HSV-1 infected cells treated with Acyclovir (antiviral drug) did not show any amplification by PCR.
Conclusions: In this preliminary study, the Indian medicinal plant extract, Swertia chirata showed antiviral properties against Herpes simplex virus type-1.

Keywords
Antiviral activity, cytotoxicity assay, herpes simplex virus, immunofluorescence, polymerase chain reaction

 
© Copyright 2008 Indian Journal of Medical Microbiology.
Alternative site location: http://www.ijmm.org

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