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Indian Journal of Medical Microbiology
Medknow Publications on behalf of Indian Association of Medical Microbiology
ISSN: 0255-0857
EISSN: 1998-3646
Vol. 26, No. 4, 2008, pp. 361-364
Bioline Code: mb08110
Full paper language: English
Document type: Short Communication
Document available free of charge

Indian Journal of Medical Microbiology, Vol. 26, No. 4, 2008, pp. 361-364

 en Validation of multiplex PCR strategy for simultaneous detection and identification of methicillin resistant Staphylococcus aureus check for this species in other resources
Rallapalli, S; Verghese, S & Verma, RS

Abstract

Multiplex polymerase chain reaction (PCR) strategy is described for rapid identification of clinically relevant methicillin resistant Staphylococcus aureus check for this species in other resources (MRSA) that targets mecA and coag ulase genes. In this study, 150 staphylococcal clinical isolates were used that included 40 isolates of MRSA, 55 isolates of methicillin susceptible S. aureus (MSSA), 44 isolates of methicillin susceptible coag ulase negative Staphylococcus spp. (MS-CoNS) and 11 isolates of methicillin resistant coag ulase negative Staphylococcus spp. (MR-CoNS). Out of 55 S. aureus strains, three strains demonstrated mecA gene, which appeared to be oxacillin sensitive by disc diffusion. When (MS-CoNS) were evaluated, 10 isolates classified as oxacillin sensitive phenotypically, yielded positive results in PCR method. The results for mecA detection by PCR were more consistent with disk susceptibility tests in case of MRSA (100%) and MSSA (95%) isolates. In contrast to above results with MRSA and MSSA, mecA detection by PCR in MS-CoNS showed less correlation with disk susceptibility tests (77%). The results for coag detection by PCR were consistent with phenotypic tests in all isolates.

Keywords
Methicillin resistance, methicillin resistant Staphylococcus aureus, polymerase chain reaction, coag gene, mecA gene.

 
© Copyright 2008 Indian Journal of Medical Microbiology.
Alternative site location: http://www.ijmm.org

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