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Indian Journal of Medical Microbiology
Medknow Publications on behalf of Indian Association of Medical Microbiology
ISSN: 0255-0857
EISSN: 0255-0857
Vol. 27, No. 2, 2009, pp. 149-152
Bioline Code: mb09044
Full paper language: English
Document type: Short Communication
Document available free of charge

Indian Journal of Medical Microbiology, Vol. 27, No. 2, 2009, pp. 149-152

 en Detection of Porcine Rotavirus from Tissue and Faecal Specimens
Prabha, Suji & Verghese, Susan

Abstract

Porcine small intestinal sub-mucosa is a cell-free collagen matrix that has demonstrated its ability as a scaffold material. Transplantation poses special hazards because grafted tissues and organs transmit pathogens efficiently, especially viruses. Rotavirus is thought to be confined to the intestine, causing acute diarrhoea. The purpose of this study was to evaluate the porcine intestinal tissue scaffold for Rotavirus and to study the incidence of this virus among pig herds. Only one isolate was successfully adapted to grow in cell line MA 104 from faecal samples. This isolate was further confirmed by reverse transcriptase polymerase chain reaction and sequence analysis.

Keywords
MA 104, Rotavirus, reverse transcriptase polymerase chain reaction

 
© Copyright 2009 Indian Journal of Medical Microbiology.
Alternative site location: http://www.ijmm.org

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