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Indian Journal of Medical Microbiology
Medknow Publications on behalf of Indian Association of Medical Microbiology
ISSN: 0255-0857
EISSN: 0255-0857
Vol. 27, No. 4, 2009, pp. 321-324
Bioline Code: mb09091
Full paper language: English
Document type: Research Article
Document available free of charge

Indian Journal of Medical Microbiology, Vol. 27, No. 4, 2009, pp. 321-324

 en Distribution of Malassezia check for this species in other resources species in patients with pityriasis versicolor in Northern Iran
Shokohi, T.; Afshar, P. & Barzgar, A.

Abstract

Purpose : Malassezia check for this species in other resources yeasts are globally distributed agents of pityriasis versicolor and are implicated in the pathogenesis of seborrhoeic and atopic dermatitis. The aim of this study is to identify the Malassezia species obtained from pityriasis versicolor patients, using morphological, biochemical, physiological as well as Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) methods.
Materials and Methods: The identification of Malassezia species is performed according to microscopic features and physiological characteristics, including catalase reaction and Tween assimilation tests. The DNA is extracted from cultured Malassezia using the glass bead, phenol-chloroform method. The internal transcribed spacer 1(ITS1) region is amplified and there is restricted digestion of the PCR products with two enzymes Cfo I and Bst F5I.
Results : The most commonly isolated species is M. globosa (47.6%). RFLP analysis of the PCR products of the ITS1 region is in complete agreement with those from the DNA sequences of the internal transcribed spacer (ITS) 1 region and the biochemical tests.
Conclusion : Based on the findings of this study, it can be concluded that PCR-RFLP is a relatively simple and quick method, completely comparable to the routine methods used for Malassezia identification.

Keywords
Malassezia, pityriasis versicolor, identification, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism

 
© Copyright 2009 Indian Journal of Medical Microbiology.
Alternative site location: http://www.ijmm.org

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