Purpose: To investigate the difference between the abilities of
Helicobacter pylori
and
Escherichia coli
to induce expression of TNF-α in human peripheral blood mononuclear cells (PBMC).
Materials and Methods: H pylori was isolated from gastric biopsy specimens. The mononuclear cells were isolated from human blood, cultured, and treated with either intact or sonicated
E coli or
H pylori, and mRNA expression for TNF-α was detected using semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR).
Results: TNF-α mRNA expression levels were significantly higher in PBMCs stimulated with
E coli compared to those stimulated with
H pylori at the same number and identical conditions (
P < .001). The results also suggest that sonicated bacteria were significantly (
P < .001) less stimulatory for PBMCs than intact bacteria for both
E coli and
H pylori.
Conclusions: The ability of different H pylori strains isolated from biopsy samples to stimulate TNF-α from PBMCs was significantly lower than that of E coli. Sonicated bacteria, as compared to intact bacteria, was a very poor inducer of TNF-α mRNA expression, suggesting that the conformation of lipopolysaccharides (LPS) on the outer leaflet of the outer membrane is not totally conserved in sonicated bacteria.
