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Indian Journal of Medical Microbiology
Medknow Publications on behalf of Indian Association of Medical Microbiology
ISSN: 0255-0857
EISSN: 0255-0857
Vol. 28, No. 3, 2010, pp. 238-240
Bioline Code: mb10071
Full paper language: English
Document type: Short Communication
Document available free of charge

Indian Journal of Medical Microbiology, Vol. 28, No. 3, 2010, pp. 238-240

 en Comparison of an in-house latex agglutination test with IgM ELISA and MAT in the diagnosis of leptospirosis
Shekatkar, S; Acharya, N S.; Harish, B N. & Parija, S C.

Abstract

The laboratory diagnosis of leptospirosis is fraught with several problems. Isolation of Leptospira check for this species in other resources by culture has a low sensitivity and the microscopic agglutination test (MAT) is time consuming To overcome these problems, a rapid latex agglutination test (LAT) has been standardized for the detection of antileptospiral antibodies in serum samples from suspected cases of leptospirosis. We compared the efficiency of the LAT to a commercially available IgM ELISA and MAT. A total of 150 serum samples were tested by LAT, IgM ELISA, and MAT. The positivity was 26.7%, 26% and 24% respectively. The sensitivity and specificity of LAT as compared to MAT was 90.62 and 91.96% respectively. Even though LAT and ELISA showed similar results, its rapidity and simplicity made latex agglutination test more suitable as a rapid screening test.

Keywords
Leptospirosis, latex agglutination test, microscopic agglutination test, IgM ELISA, diagnosis

 
© Copyright 2010 Indian Journal of Medical Microbiology.
Alternative site location: http://www.ijmm.org

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