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Indian Journal of Medical Microbiology
Medknow Publications on behalf of Indian Association of Medical Microbiology
ISSN: 0255-0857 EISSN: 0255-0857
Vol. 28, No. 4, 2010, pp. 376-379
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Bioline Code: mb10110
Full paper language: English
Document type: Short Communication
Document available free of charge
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Indian Journal of Medical Microbiology, Vol. 28, No. 4, 2010, pp. 376-379
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Comparison of isoelectric focusing and polymerase chain reaction for the detection of β-lactamases
Sharma, J.; Ray, P.; Sharma, M. & Ghosh, S.
Abstract
Extended spectrum β-lactamases (ESBLs) have been observed in virtually all the species of family Enterobacteriaceae. Threat posed by antibiotic resistance because of ESBLs is more serious as a number of technical problems are associated with the detection of these enzymes. Although a number of detection methods have been designed for ESBLs, every method has its own benefits and shortcomings as well. In earlier days, isoelectric focusing (IEF) was used as the gold standard for ESBL detection. This study was undertaken to compare IEF with polymerase chain reaction, a method which has been extensively used for ESBL detection these days.
Keywords
Extended spectrum β-lactamase, isoelectric focusing, isoelectric point
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© Copyright 2010 Indian Journal of Medical Microbiology. Alternative site location: http://www.ijmm.org
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