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Indian Journal of Medical Microbiology
Medknow Publications on behalf of Indian Association of Medical Microbiology
ISSN: 0255-0857
EISSN: 0255-0857
Vol. 28, No. 4, 2010, pp. 380-384
Bioline Code: mb10111
Full paper language: English
Document type: Short Communication
Document available free of charge

Indian Journal of Medical Microbiology, Vol. 28, No. 4, 2010, pp. 380-384

 en Nosocomial outbreak of septicaemia in neonatal intensive care unit due to extended spectrum β-lactamase producing Klebsiella pneumoniae check for this species in other resources showing multiple mechanisms of drug resistance
Rastogi, V.; Nirwan, P. S.; Jain, S. & Kapil, A.

Abstract

A total of 14 phenotypically similar clinical isolates of Klebsiella pneumoniae check for this species in other resources , resistant to multiple drugs including cefotaxime and ceftazidime, were isolated from blood of neonates admitted to neonatal intensive care unit (NICU) within a short span of 10 days. Alarmed at the possibility of occurrence of outbreak, a thorough investigation was done. Microbiological sampling of the NICU and labour room (LR) environment yielded 12 K. pneumoniae isolates. The presence of extended spectrum β-lactamase (ESBL) in the clinical and environmental strains was detected by double-disk synergy test (DDST), CLSI phenotypic confirmatory disk diffusion test (PCDDT) and E-test ESBL strips. Amp-C screen (disk) test was done to determine Amp-C β-lactamase production. 100% clinical strains, 57% NICU strains and 80% LR strains were ESBL positive. 57% clinical, 43% NICU and 20% LR strains were Amp-C screen positive. Polymerase chain reaction (PCR) of representative ESBL positive (10 clinical and 5 environmental) strains showed CTX gene and TEM and/or SHV gene in all. K. pneumoniae showing multiple mechanisms of drug resistance was responsible for the outbreak.

Keywords
Extended spectrum β-lactamase, Klebsiella pneumoniae, neonatal septicaemia, outbreak

 
© Copyright 2010 Indian Journal of Medical Microbiology.
Alternative site location: http://www.ijmm.org

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