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Indian Journal of Medical Microbiology
Medknow Publications on behalf of Indian Association of Medical Microbiology
ISSN: 0255-0857
EISSN: 0255-0857
Vol. 29, No. 1, 2011, pp. 42-46
Bioline Code: mb11010
Full paper language: English
Document type: Research Article
Document available free of charge

Indian Journal of Medical Microbiology, Vol. 29, No. 1, 2011, pp. 42-46

 en Comparison of four methods for rapid identification of Staphylococcus aureus check for this species in other resources directly from BACTEC 9240 blood culture system
Ozen, N.S.; Ogunc, D.; Mutlu, D.; Ongut, G.; Baysan, B.O. & Gunseren, F.


Purpose: Differentiation of Staphylococcus aureus (S. aureus) from coagulase-negative staphylococci is very important in blood stream infections. Identification of S. aureus and coagulase-negative staphylococci (CoNS) from blood cultures takes generally 18-24 h after positive signaling on continuously monitored automated blood culture system. In this study, we evaluated the performance of tube coagulase test (TCT), slide agglutination test (Dry Spot Staphytect Plus), conventional polymerase chain reaction (PCR) and LightCycler Staphylococcus MGrade kit directly from blood culture bottles to achieve rapid identification of S. aureus by using the BACTEC 9240 blood culture system.
Materials and Methods: A total of 129 BACTEC 9240 bottles growing gram-positive cocci suggesting Staphylococci were tested directly from blood culture broths (BCBs) with TCT, Dry Spot Staphytect Plus, conventional PCR and LightCycler Staphylococcus MGrade kit for rapid identification of S. aureus.
Results: The sensitivities of the tests were 99, 68, 99 and 100%, respectively.
Conclusion: Our results suggested that 2 h TCT was found to be simple and inexpensive method for the rapid identification of S. aureus directly from positive blood cultures.

Blood culture system, direct identification, polymerase chain reaction, S. aureus, slide agglutination test

© Copyright 2011 Indian Journal of Medical Microbiology.
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