THERMOSTABILITY OF RECONSTITUTED NEWCASTLE DISEASE VIRUS STRAINS AT 36°C TEMPERATURE

Haemagglutination (HA) test was employed to determine the stability of HA titers of reconstituted form of Hitchner - B1 (B1), LaSota (L) and Komarov (K) strains of Newcastle Disease Vaccine (NDV) at 36°c. The temperature treatment method was through incubation (in water bath) of the reconstituted vaccines at selected temperature and sequential sampling of each vaccine vial for the determination of pre - and post - temperature exposure HA titers. Thus, on the basis of a two-step (2log₂) decline in titer as evidence of loss of stability of HA titers (LST), the LST therefore, occurred at 50^th, 24^th and 95^th hour for BI, L and K strains, respectively, post - temperature exposure. The data, therefore, showed that the NDV - K strain was the most stable at the test temperature. It is believed that the findings will enhance the understanding of the potential of this strain in the developed and application of a thermostable MDV target on village poultry. In rural settings.

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Keywords
Haemagglutination, Thermostability, Newcastle Disease Virus, Reconstituted and Strain.