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African Journal of Biomedical Research
Ibadan Biomedical Communications Group
ISSN: 1119-5096
Vol. 6, No. 1, 2003, pp. 37-42
Bioline Code: md03007
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Biomedical Research, Vol. 6, No. 1, 2003, pp. 37-42

 en UTILIZATION OF CORN STARCH AS SUBSTRATE FOR β-AMYLASE BY BACILLUS SPP
AJAYI A.O AND FAGADE O.E

Abstract

Corn starch was used as substrate for β -amylase production from ten(10) amylolytic species of the genus Bacillus check for this species in other resources isolated locally from soil, waste water and food sources. Ten bacillus strains was made up of two strains each of Bacillus macerans check for this species in other resources , Bacillus licheniformis check for this species in other resources and Bacillus circulans check for this species in other resources . Also included are B. coagulans, B. megatarium, B. polymyxa and B. subtilis. B. cereus ATCC 11778 served as type specie. The mean estimate of total bacterial count for all the amylolytic bacillus strains studied was 11.58 X 10 2 CFU/ml in corn starch substrate, 10.1 X10 2 CFU/ml soluble starch and nutrient broth medium recorded 7.4 X 10 2 CFU/ml. The growth value expressed in corn starch substrates shows its viability as a cheap carbon source. β-amylase production by the various strains showed that B. licheniformis (S2) (4.2 unit/ml) and B. subtilis (6.24 unit/ml) has high enzymatic activity with corresponding maltose yield of 46.4mg/ml and 68.0mg/ml respectively. Calcium ion added to the assay systems improved β-amylase activity.

 
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