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African Journal of Biomedical Research
Ibadan Biomedical Communications Group
ISSN: 1119-5096
Vol. 10, No. 2, 2007, pp. 117-125
Bioline Code: md07016
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Biomedical Research, Vol. 10, No. 2, 2007, pp. 117-125

 en A Comparative Assessment of Ribosomal DNA Polymorphisms in Methicillin Resistant Staphylococcus Aureus check for this species in other resources (MRSA) Epidemiology
Terry Alli, O.A.; Akinloye, O.; Rowley, D.A. & Butcher, Philip D.


Chromosomal DNA from 19 strains of methicillin resistant Staphylococcus Aureus check for this species in other resources (MRSA) which included 13 strains from different geographical locations in England and 6 strains from St George’s Hospital (SGH), London, with MRSA NCTC 119040 and Oxford S. aureus were digested with the restriction endonucleases, HindIII and HaeIII. Southern blot hybridisation was carried out on the DNA digests transferred onto membrane using radioactive probe prepared from 16S and 23S rRNA from Escherichia coli check for this species in other resources . The pattern of bands which depended on restriction fragment length polymorphisms was used as a measure of minor genomic variation within the MRSA strains. Phage typing, biotyping, antibiogram, and plasmid profile analysis were also carried out on all the strains for comparison. Four and five different patterns were obtained for HindIII and HaeIII, respectively for representative isolates from different geographical locations in England for that year. Three different patterns were identified among the MRSA strains from SGH, London, using HaeIII. None of the epidemiological markers used in this study gave results that correlated with each other. Ribosomal DNA polymorphisms have proved to be a very useful technique for studying the epidemiology of MRSA in hospitals when used along with other epidemiological markers.

MRSA, rDNA polymorphism, biotyping , phage typing, antibiogram, plasmid profile analysis..

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