Bacterial Isolates of different Genera were collected from suspected cases of Urinary tract infection in Ibadan and their prevalence was determined. Sensitivity pattern of the organisms to quinolone antibacterial agents was also investigated by the antibiotic disc diffusion method using Kirby Bauer method. The study revealed the prevalence of uropathogenic organisms as follows:- Escherichia coli
spp (23.1%), Staphylococcus aureus
(21.1%) and Pseudomonas aeruginosa
(9.6%). The quinolones were found to be highly effective against all the organisms. The average percentage susceptibility of the organisms were as follow: Staphylococcus aureus
(92.7%) Escherichia coli
(81.7%), Pseudomonas aeruginosa
(76.0%), and Klebsiella
spp (70.0%). As indicated by their high activity, quinolones are better alternatives to commonly prescribed antibiotics in Urinary tract infection therapy although caution must be exercised in their prescription as the emerging low level of resistance may portend great danger for their future use.