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African Journal of Biomedical Research
Ibadan Biomedical Communications Group
ISSN: 1119-5096
Vol. 11, No. 1, 2008, pp. 55-63
Bioline Code: md08008
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Biomedical Research, Vol. 11, No. 1, 2008, pp. 55-63

 en Free Radical Scavenging Activity of Crude Extracts and 4´-O-Methylepigallocatechin Isolated From Roots of Cassine Transvaalensis Burtt-Davy from Botswana
Motlhanka, D.M.T; Habtemariam, S & Houghton, P


Water and ethanol extracts of roots from Cassine transvaalensis check for this species in other resources Burtt-Davy (celastraceae) were assessed for in vitro antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay. The ethanolic extract exhibited higher free radical scavenging effect than the water extract at all tested concentrations. Above 100µg/ml, the ethanolic extract showed 80% scavenging activity, similar to control antioxidant compounds quercetin, rutin and L-ascorbic acid. The water extract reached a similar level of activity (80%) at 200µg/ml. Between 20-50µg/ml, 4'-O-methyl-epigallocatechin isolated by bioassay directed fractionation exhibited scavenging activity greater than that of either the ethanolic or aqueous crude extract. However, at concentrations above 50µg/ml, the scavenging activity of the ethanolic extract exceeded that of 4'-O-methyl-epigallocatechin.The results suggest that extracts from the roots of Cassine transvaalensis have strong antioxidant activity. These findings support the ethnomedical use of this plant to promote good health.

Cassine transvaalensis; antioxidant; DPPH radicals; 4’-O-methyl-epigallocatechin; epicatechin

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