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African Journal of Biomedical Research
Ibadan Biomedical Communications Group
ISSN: 1119-5096
Vol. 11, No. 1, 2008, pp. 55-63
Bioline Code: md08008
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Biomedical Research, Vol. 11, No. 1, 2008, pp. 55-63

 en Free Radical Scavenging Activity of Crude Extracts and 4´-O-Methylepigallocatechin Isolated From Roots of Cassine Transvaalensis Burtt-Davy from Botswana
Motlhanka, D.M.T; Habtemariam, S & Houghton, P

Abstract

Water and ethanol extracts of roots from Cassine transvaalensis check for this species in other resources Burtt-Davy (celastraceae) were assessed for in vitro antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay. The ethanolic extract exhibited higher free radical scavenging effect than the water extract at all tested concentrations. Above 100µg/ml, the ethanolic extract showed 80% scavenging activity, similar to control antioxidant compounds quercetin, rutin and L-ascorbic acid. The water extract reached a similar level of activity (80%) at 200µg/ml. Between 20-50µg/ml, 4'-O-methyl-epigallocatechin isolated by bioassay directed fractionation exhibited scavenging activity greater than that of either the ethanolic or aqueous crude extract. However, at concentrations above 50µg/ml, the scavenging activity of the ethanolic extract exceeded that of 4'-O-methyl-epigallocatechin.The results suggest that extracts from the roots of Cassine transvaalensis have strong antioxidant activity. These findings support the ethnomedical use of this plant to promote good health.

Keywords
Cassine transvaalensis; antioxidant; DPPH radicals; 4’-O-methyl-epigallocatechin; epicatechin

 
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