search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


African Journal of Biomedical Research
Ibadan Biomedical Communications Group
ISSN: 1119-5096
Vol. 11, No. 2, 2008, pp. 173-181
Bioline Code: md08023
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Biomedical Research, Vol. 11, No. 2, 2008, pp. 173-181

 en The Mycobacterium tuberculosis check for this species in other resources homologue of the Mycobacterium avium check for this species in other resources mig gene is not specifically expressed in the macrophage
Terry, Alli O .A.; Hingley-Wilson, S.; Ogbolu, D.O. & Spreadbury, Claire L.

Abstract

With the completion of genome sequencing of Mycobacterium tuberculosis check for this species in other resources and upsurge in the incidence of M. tuberculosis infection worldwide partly as a result of HIV pandemic, there is need for rationale approach to vaccine and chemotherapy discoveries for M. tuberculosis. The homologue of mig gene of Mycobacterium avium check for this species in other resources was searched for in the M. tuberculosis database at The Institute of Genomic Research (TIGR), USA and The Sanger Institute, UK. Homologue of the gene was found and comprehensively analysed. Reverse transcription PCR (RT-PCR) was carried out on the mig (fadD19) gene homologue and echA19 gene. The result of the RT-PCR showed that the mig gene was at least 2-fold upregulated during intracellular infection of macrophage compared to the broth grown bacilli as opposed to the demonstrated specific expression of mig gene in M. avium infected macrophage. The echA19 gene was also found to be upregulated. .

Keywords
macrophage induced gene, mig gene, Mycobacterium tuberculosis

 
© Copyright 2008 - Ibadan Biomedical Communications Group

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 16-Oct-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil