With the completion of genome sequencing of Mycobacterium tuberculosis
and upsurge in the incidence of M. tuberculosis
infection worldwide partly as a result of HIV pandemic, there is need for rationale approach to vaccine and chemotherapy discoveries for M. tuberculosis
. The homologue of mig gene of Mycobacterium avium
was searched for in the M. tuberculosis
database at The Institute of Genomic Research (TIGR), USA and The Sanger Institute, UK. Homologue of the gene was found and comprehensively analysed. Reverse transcription PCR (RT-PCR) was carried out on the mig
) gene homologue and echA19 gene. The result of the RT-PCR showed that the mig gene was at least 2-fold upregulated during intracellular infection of macrophage compared to the broth grown bacilli as opposed to the demonstrated specific expression of mig gene in M. avium
infected macrophage. The echA19 gene was also found to be upregulated. .