search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


African Journal of Biomedical Research
Ibadan Biomedical Communications Group
ISSN: 1119-5096
Vol. 12, No. 1, 2009, pp. 73-76
Bioline Code: md09013
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Biomedical Research, Vol. 12, No. 1, 2009, pp. 73-76

 en Comparison of the Fluorescent Antibody Test and Direct Microscopic Examination for Rabies Diagnosis at the National Veterinary Research Institute, Vom, Nigeria.
Ehizibolo, D.O.; Nwosuh, C.I.; Ehizibolo, E.E. & Kia, G.S.N.

Abstract

One hundred and eighty-nine (189) dog brain samples submitted to the National Veterinary Research Institute, Vom, Nigeria for rabies diagnosis were assayed by the Direct Microscopic Examination (DME) for Negri bodies, the Fluorescent Antibody Test (FAT) for rabies antigens, and the Mouse Inoculation Test (MIT). The MIT was used as a confirmatory test. There were 12(12.5%) false negative and 6(6.7%) false positive results with DME, while there was 2(1.7%) false negative and 2(1.7%) false positive with FAT. The FAT was more sensitive (98.26%) than the DME (87.5%), and also more specific, 97.29% and 93.55% respectively. The FAT predicted positive and negative results more accurately than the DME. Positive predictive value of FAT was 98.26% compared with 93.33% of DME, and the negative predictive value of FAT was 97.29% compared with DME’s 87.88%. The pre-test probability of rabies in Vom was 60.8%. This finding shows that FAT is a sensitive and reliable rabies diagnostic test than DME and its continued use is recommended in Nigeria whenever is feasible. The DME is still useful as a screening test, and the MIT must be used to confirm both FAT and DME

Keywords
Fluorescent antibody test, mouse inoculation, direct microscopic examination, rabies diagnosis, Nigeria.

 
© Copyright 2009 - African Journal of Biomedical Research

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 16-Oct-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil