search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


African Journal of Biomedical Research
Ibadan Biomedical Communications Group
ISSN: 1119-5096
Vol. 14, No. 2, 2011, pp. 143-146
Bioline Code: md11023
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Biomedical Research, Vol. 14, No. 2, 2011, pp. 143-146

 en Osmotic Fragility in Stored Non-Detergent Washed Human Erythrocytes
Okwusidi, John I.

Abstract

Osmotic fragility (OF) of non-detergent washed erythrocytes was evaluated in Nigerian human male erythrocytes stored for 0h, 12h, 24h and 48h. Storage of these human erythrocytes for up to 24h did not significantly alter their membrane characteristics. Mean corpuscular fragility (MCF) diminished and correlated negatively (r = -0.9668) with storage time. Only the 48h storage-time MCF exhibited significant variance compared to 0h, 12h and 24h (p <0.001). These results support OF-test data as reliable indices of alteration of membrane characteristics of non-detergent washed human erythrocytes stored for up to 24h. Non-detergent washing enhances erythrocytes OF test viability and hence the time window for meaningful OF test especially in situations of large sample volume.

Keywords
Osmotic fragility; Stored washed human erythrocytes; Mean corpuscular fragility

 
© Copyright 2011 - African Journal of Biomedical Research

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 16-Oct-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil