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African Journal of Biomedical Research
Ibadan Biomedical Communications Group
ISSN: 1119-5096
Vol. 16, No. 1, 2013, pp. 19-24
Bioline Code: md13003
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Biomedical Research, Vol. 16, No. 1, 2013, pp. 19-24

 en Phytosterols from Spondias mombin check for this species in other resources Linn with Antimycobacterial Activities
Olugbuyiro, J. A. O,; Moody, J. O & Hamann, M. T

Abstract

The growing problems of tuberculosis have led to the search for new anti-Mtb agents from higher plants. The stem bark of Spondias mombin check for this species in other resources was evaluated for its in vitro activity against Mycobacterium tuberculosis check for this species in other resources (H37Rv strain). Bioassay-guided fractionation of the methanol extract was carried out by Vacuum Liquid Chromatography (VLC) on Silica gel (230-400 mesh) and purification was done using HPLC and TLC. In vitro antimycobacterial susceptibility was performed by a fluorometric microplate alamar blue assay (MABA) and percentage mycobacterial inhibition was calculated. The structures of the isolated compounds were established by spectroscopic analysis. The active VLC fraction exhibited 91% inhibition against M. tuberculosis H37Rv at a concentration of 40 μg/mL. The HPLC fraction SMi-15 containing compounds 1 and 2 showed 92.8% inhibition against M. tuberculosis. Two new antimycobacterial phytosterols were isolated from the stem bark of S. mombin and the structureswere identified as mombintane I (1) and mombintane II (2). The stem bark extractives of S. mombin contain antitubercular principles of the class phytosterol and support an important potential of triterpenoids.

Keywords
Spondias mombin; Mycobacterium tuberculosis; antimycobacterial; mombintane I; mombintane II

 
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