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Effects of Intestinal Ischaemia-Reperfusion Injury and Splenectomy on Haematological and Biochemical Parameters in Rats Olatunji-Akioye, A.O & Fayemi, O Abstract Splenectomy has been proposed to alleviate mild and sustained ischemia reperfusion injury (IR). Intestinal ischaemia reperfusion injury (IIR) appears to have remote effects from the gastrointestinal tract. The effects of splenectomy on haematology and serum biochemical parameters in mild and sustained intestinal ischemia reperfusion injury was evaluated in male Wistar rats. The rats were splenectomized and then subjected to either 20minutes or one hour of superior mesenteric artery and collateral supply ischemia and one hour of reperfusion. Control rats were subjected to either 20minutes or one hour of ischemia and one hour of reperfusion only. Blood samples were collected before and after surgery for control animals and the other groups, for analysis. Mild IIR significantly reduced packed cell volume (32.4±2.6%), haemoglobin concentration (9.7±0.1g/dL), red blood cell count (5.3±0.3X10⁶/L) and leucocytosis (16.3±0.28X103/μL) due to lymphocytosis (78%), postoperatively. Sustained IIR significantly lowered values of neutrophils (29%). Mild and sustained IIR did not significantly alter biochemical parameters. Significantly lower albumin values were observed in sustained IIR (3.4±0.55g/dL). Splenectomy did not significantly alter hematological parameters, a measure of protection. Elective splenectomy may thus be useful in moderating haematological changes that may occur following an incident in which intestinal ischaemia-reperfusion injury may follow as a palliative measure. Keywords splenectomy; haematology; serum chemistry; Wistar rats; intestinal ischaemia-reperfusion injury

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