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African Journal of Biomedical Research
Ibadan Biomedical Communications Group
ISSN: 1119-5096
Vol. 17, No. 3, 2014, pp. 165-171
Bioline Code: md14024
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Biomedical Research, Vol. 17, No. 3, 2014, pp. 165-171

 en Antimicrobial Activity of Garcinia kola check for this species in other resources (Heckel) Seed Extracts and Isolated Constituents Against Caries-causing Microorganisms.
Ajayi, T.O.; Moody, J. O.; Fukushi, Y.; Adeyemi, T. A. & Fakeye, T. O.


Garcinia kola check for this species in other resources Heckel (Guttiferae) seed, has found use in folkloric medicine in Southern Nigeria for the treatment of toothache and prevention of dental caries. We have evaluated the crude ethanolic extract, chromatographic fractions and isolated constituents of this Nigerian edible seed against clinical strains of dental-caries-causing and related microorganisms. Antimicrobial evaluation was done by testing different concentrations of the crude extract, vacuum liquid chromatographic (VLC) fractions and pure isolates against Streptococcus mutans check for this species in other resources , Streptococcus viridans and Staphylococcus aureus check for this species in other resources in already set blood agar with gentamicin as the reference standard. The zones of inhibition and minimum inhibitory concentrations (MIC) were determined as appropriate. Fraction N, eluted with (hexane: ethyl acetate 70: 30), exhibited the highest activity with MIC’s of 1.50 mgml⁻¹ and 0.33 mgml⁻¹ while the pure isolates 1 (cycloartenol) and 2 (24-methylenecycloartanol) gave MIC’s of 0.17 mgml⁻¹ and 0.38 mgml⁻¹ against Streptococcus mutans and Streptococcus viridans respectively. Isolate 3 (garcinianin) gave MIC of 1.0 mgml⁻¹ against Streptococcus mutans but no significant activity against Streptococcus viridans and Staphylococcus aureus. The results provide some justifications for the folkloric use of Garcinia kola Heckel (Guttiferae) for dental caries-related health problems while the isolated compounds may also serve as templates for future drug development.

Garcinia kola; 24-methylenecycloartanol; cycloartenol; Streptococcus; garcinianin

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