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African Journal of Biomedical Research
Ibadan Biomedical Communications Group
ISSN: 1119-5096
Vol. 19, No. 3, 2016, pp. 241-247
Bioline Code: md16034
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Biomedical Research, Vol. 19, No. 3, 2016, pp. 241-247

 en Protective Effects of Aqueous Extract of Telfairia occidentalis check for this species in other resources on Mercury-Induced Histological and Oxidative Changes in the Rat Hippocampus and Cerebellum
Owoeye, O. & Gabriel, M.O.

Abstract

Mercury intoxication in rodents causes damage to various organs including the brain via oxidative stress. Aqueous extract of Telfairia occidentalis check for this species in other resources (TOAE) may be a preventive agent by virtue of its reported antioxidant property. The present study was carried out to investigate the possible protective role of TOAE against mercuric chloride (HgCl2)-induced changes in rat brain. Twenty-four adult female rats (150g-200g) were randomized into four groups of six rats each after acclimatization viz: I: Control, tap water; II: TOAE (400 mg/kg body wt.); III: HgCl2 (4 mg/kg body wt.); IV: TOAE (400 mg/kg body wt.) + HgCl2 (4 mg/kg body wt.). All treatments were oral by gastric gavage and lasted 14 days. Behavioural tests were conducted on the 15th day after which rats were euthanized with i.p Ketamine (100mg/kg) same day. Brain weight, antioxidant parameters [malondialdehyde (MDA), glutathione (GSH), catalase (CAT) and super oxide dismutase (SOD)], behavioural studies, and histology of rat hippocampus and cerebellum with regard to micro-anatomical parameters were examined in all groups. Mercuric chloride (HgCl2) induced significant (p<0.0.05) elevation of the MDA level, activities of CAT and SOD, grooming and locomotion frequency but reduced GSH level relative to control. Also, it induced the death of granule cells, pyramidal cells and Purkinje neurons thus altering the microanatomy of these brain structures. However, concomitant administration of TOAE with HgCl2 caused a reversal of these parameters relative to HgCl2.–treated rats. In conclusion, aqueous extract of Telfairia occidentalis demonstrated protective effects against HgCl2-induced oxidative and histological changes of the microanatomy of rat hippocampus and cerebellum.

Keywords
Mercuric chloride; Telfairia occidentalis; hippocampus; cerebellum; antioxidant

 
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