Phytochemical analysis of Aqueous Methanolic Extract of Acanthospermum hispidium and its effect on Biochemical and Hematological indices in Plasmodium falciparum infected Rats|
Azeez, T.O & Banigo, A.T
Haematological (Red blood cell ‘RBC’, packed cell volume ‘PCV’, white blood cell ‘WBC’ and platelet ‘PLA’) and biochemical
(total protein, serum albumin and creatinine) parameters of rats treated with aqueous-methanolic extract of A. hispidum leaves
on blood serum of rats infected with P. falciparum was investigated. The chemical composition was determined using gas
chromatography and mass spectrometry (GC-MS). Biochemical and haematological parameters were examined using standard
methods. The aqueous-methanolic extract of A. hispidum consist of Allyldimethyl-Formamide, Erythritol, Glycerin,
Benzoylmethyl-3-hydroxy-5-nitro-2-indolinone, Silanol and Hexadecanoic acid, 1-(hydroxymethyl)-1,2-ethanediyl ester with
composition of 13.578, 2.796, 56.72, 5.486, 1.415 and 10.005%, respectively. The increase in RBC, PCV, PLA and albumin
with reduction in WBC and creatinine level compared to normal control when treated with aqueous – methanolic extract of A.
hispidum leaves were obtained with reduced parasitaemia. Aqueous – methanolic A. hispidum leaves, at 400mg/kg for 72 hours
proved to be the best for treatment of malaria parasite based on haematological and biochemical parameters assessed.
Acanthospermum hispidum; Plasmodium falciparum; GC- MS; haematological and Biochemical parameters