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Medicina
Croatian Medical Association - Rijeka branch
ISSN: 0025-7729
Vol. 43, No. 1, 2007, pp. 47-54
Bioline Code: me07007
Full paper language: Croatian
Document type: Research Article
Document available free of charge

Medicina, Vol. 43, No. 1, 2007, pp. 47-54

 hr Određivanje Otpornosti Vrste Mycobacterium Tuberculosis Na Izoniazid Multipleks Pcr Metodom
Obrovac, Mihaela; Katalinić-Janković, Vera & Grce, Magdalena


Cilj: Ispitati mutacije u katG i inhA genima kliničkih izolata vrste Mycobacterium tuberculosis check for this species in other resources (M. tuberculosis), otpornih na izoniazid (INH).
Metode: Za to su ispitivanje izdvojena ukupno 103 klinička izolata vrste M. tuberculosis, prikupljena u razdoblju od 1999. do 2006. na području Hrvatske, kojima je fenotipskom metodom proporcije prema Canettiju, na Löwenstein-Jensen podlozi, utvrđena otpornost na INH. Ispitivanje mutacija u katG i inhA genima, provedeno je multipleks PCR metodom. Ta se metoda zasniva na otkrivanju mutacije AGC→ACC u katG genu, te inhAC-15T mutacije u regulatornoj regiji mabA-inhA operona.
Rezultati: Za 76 sojeva (75%) od ukupno 103 ispitivana soja vrste M. tuberculosis, utvrđena je genetska osnova otpornosti na INH. Ustanovljena je povezanost između mutacije u kodonu 315 katG gena, te razvoja otpornosti na druge antituberkulotike.
Zaključak: Potrebno je ispitati genetske osnove otpornosti na INH, jer se katG mutacija pokazuje kao pretkazivač mogućega razvoja otpornosti na druge antituberkulotike.


multipleks PCR; rezistentna tuberkuloza; izoniazid; ispitivanje osjetljivosti

 
 en Determination Of The Resistance Of Mycobacterium Tuberculosis Against Isoniazid By Multiplex Pcr Method
Obrovac, Mihaela; Katalinić-Janković, Vera & Grce, Magdalena

Abstract

Aim: To detect mutations in katG and inhA genes in Mycobacterium tuberculosis check for this species in other resources (M. tuberculosis) clinical isolates resistant to isoniazid (INH).
Methods: A total of 103 isolates collected from 1999 to 2006 in Croatia and identified as INH-resistant M. tuberculosis strains by standard susceptibility testing proportion method according to Canetti were investigated. Multiplex PCR method was used to detect an AGC to ACC mutation in the katG gene and an inhAC-15T mutation in the regulatory region of the mabA-inhA operon.
Results: Genetic basis of INH resistance was found for 76 (75%) of total 103 M. tuberculosis strains investigated. There was a correlation between mutation in 315 codon of katG gene and acquisition of resistance to other antituberculous drugs.
Conclusions: Mutation in katG gene in INH resistant M.tuberculosis strains is highly predictive for acquisition of resistance to other antituberculous drugs.

Keywords
multiplex PCR; drug resistant tuberculosis; isoniazid; susceptibility testing

 
© © 2007 - Croatian Medical Association - Rijeka branch
Alternative site location: http://hrcak.srce.hr/medicina

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