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Usporedba Pokretljivosti Sjemena i Oplodnje In Vitro Tramišak-Milaković, Tamara & Ljiljak, Dejan Cilj: Cilj ovog rada bio je ispitati povezanost kompjutorske analize spermija (engl. computer-aided sperm analysis, CASA), posebno parametara koncentracije i pokretljivosti progresivnih spermija, s postizanjem oplodnje in vitro. Metode: Analiza kvalitete sjemena napravljena je pomoću CASA sistema. Analizirano je 36 bolesnika kod kojih je napravljena in vitro oplodnja (engl. in vitro fertilization, IVF) i embrio transfer (ET). Karakteristike spermija prije i nakon 'swim up' metode pročišćavanja uspoređene su između 23 ciklusa s oplodnjom ≤ 50% i 13 ciklusa bez oplodnje. Rezultati i zaključak: Ispitivanje pokazuje da parametri brzine i pokretljivosti spermija iz nativnog sjemena i spermija pročišćenih 'swim up' metodom koreliraju s oplodnjom in vitro, što ukazuje da ovi parametri mogu biti dobar pokazatelj ishoda oplodnje. kompjuterska analiza spermija, pokretljivost spermija, oplodnja in vitro

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Comparison of Sperm Motility and Fertilisation In Vitro Tramišak-Milaković, Tamara & Ljiljak, Dejan Abstract Aim: The aim of this study was to investigate the relationships between computer-aided sperm analysis (CASA), especially the concentration and movement characteristics of progressively motile spermatozoa and fertilization rates in vitro. Methods: Analysis of semen quality was performed using CASA in 36 in vitro fertilization – embryo transfer (IVF-ET) cycles with at least 2 oocytes collected. Sperm quality before and after swim-up procedure was compared between 23 cycles with fertilization rate ≤50% and 13 cycles without fertilization. Results and conclusion: Our study demonstrated that velocity parameters and motility characteristics of sperms from freshly obtained semen and those after swim up procedure correlated with fertilization rates, indicating that these parameters could be good predictors of the fertilization outcome. Keywords Computer-aided sperm analysis, sperm motility, fertilization in vitro

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