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Izražaj koštanog morfogenetskog proteina -2 i -7 tijekom eksperimentalne upalne bolesti crijeva Marić, Ivana; Kučić, Natalia; Grahovac, Blaženka; Bobinac, Dragica & Vukičević, Slobodan Cilj: Za koštane morfogenetske proteine -2 i -7 pokazano je da osim snažnog osteoinduktivnog svojstva imaju važnu ulogu u razvoju i cijeljenju različitih neskeletnih tkiva i organa. Svrha ovog rada je ispitati ekspresiju BMP-2 i BMP-7 tijekom upalne bolesti crijeva u štakora, te učinak dvotjednog liječenja sa BMP-7 na cijeljenje tkiva kolona i ekspresiju ovih morfogenetskih proteina. Metode: Eksperimentalni kolitis izazvan je primjenom klizme koja sadrži 2,4,6-trinitrobenzensulfoničnu kiselinu (TNBS) i etanol. Na uzorcima crijeva pratili smo makroskopske i mikroskopske promjene koje se zbivaju tijekom akutne i kronične faze kolitisa, te nakon i.v. primjene BMP-7. Metodom imunohistokemije i lančane reakcije polimeraze pratili smo izražaj BMP-2 i -7. Rezultati: Imunohistokemijska i RT-PCR analiza pokazala je ekspresiju BMP-2 i -7 tijekom akutne i kronične faze upalne bolesti crijeva. U akutnoj fazi kolitisa BMP-2 i -7 prisutni su u području ulkusa, a u kroničnoj fazi BMP-7 pozitivno bojanje prisutno je u kolonocitima uzduž kripti kolona, dok je BMP-2 pozitivno bojanje u kolonocitima površnog epitela te između kripta. Specifično bojanje na BMP-7 kao i ekspresija mRNA za BMP-7 na uzorcima crijeva životinja liječenim sa BMP-7 pokazuje smanjenje prisustva BMP-7, a pojačano bojanje i ekspresiju mRNA za BMP-2. Zaključak: Sistemska primjena koštanog morfogenetskog proteina-7 smanjuje makroskopsko i mikroskopsko oštećenje kolona tijekom upalne bolesti crijeva u štakora. Način ekspresije BMP-2 i -7 tijekom upalne bolesti crijeva te nakon liječenja sa BMP-7 ukazuje na značajnu ulogu koštanih morfogenetskih proteina u kontroli oštećenja i cijeljenja tkiva kolona tijekom ove bolesti. imunohistokemija, koštani morfogenetski protein-2, koštani morfogenetski protein-7, lančana reakcija polimeraze, upalna bolest crijeva

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Expression of bone morphogenetic protein -2 and -7 during experimental inflammatory bowel disease Marić, Ivana; Kučić, Natalia; Grahovac, Blaženka; Bobinac, Dragica & Vukičević, Slobodan Abstract Aim: Bone morphogenetic proteins -2 and -7, along with their osteoinductive function, play important role in the development and healing of different non-skeletal tissues and organs. The aim of this study was to reveal the expression of BMP-2 and BMP-7 during experimental inflammatory bowel disease, as well as after 14th day of BMP-7 treatment. Methods: Experimental colitis was induced by the intracolonic administration of the clysma containing 2,4,6-trinitrobenzensulphonic acid (TNBS) and ethanol. We investigated the macroscopic and microscopic colon tissue damage during acute and chronic phases of colitis as well as after IV treatment of BMP-7. Results: Imunohistochemical and RT-PCR analyses showed expression of BMP-2 and -7 during acute and chronic phases of inflammatory bowel disease. In the acute phase of colitis, BMP-2 and -7 expressions were located in mucosa of ulcer region. In the chronic phase BMP-7 expression was placed in the colonocytes along the crypt, while BMP-2 expression was located in the coloncytes of surface epithelium and intercrypt table. BMP-7 treatment reduced the expression of BMP-7 and mRNA for BMP-7 but also increased the expression of BMP-2 and mRNA for BMP-2 in colonic tissue. Conclusions: Systemic administration of bone morphogenetic protein 7 decreased macroscopic and microscopic colonic tissue damage during experimental inflammatory bowel disease. The expression pattern of BMP-2 and -7 during inflammatory bowel disease, as well as after BMP-7 treatment suggests an important role of bone morphogenetic proteins in control of colon tissue damage and healing during disease. Keywords bone morphogenetic protein-2, bone morphogenetic protein -7, immunohistochemistry, inflammatory bowel disease, polymerase chain reaction

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