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Quality and safety assurance of bone grafts stored by deep freezing method Legović, Dalen; Šantić, Veljko; Gulan, Gordan; Jurdana, Hari; Tudor, Anton; Prpić, Tomislav & Šestan, Branko Abstract Bone bank is a complex system which unifies collection, storage and distribution of bone grafts. In order to adequately use bone graft in bone and joint surgery, it must be tested microbiologically monitored and marked when ready for use with the accompanying documentation. Collection of bone grafts is carried out either from the living donors, who are giving away parts removed from the femur head during routine procedure of mounting total hip endoprosthesis or from cadaver within multiorgan explantation. Potential providers give their consent to the donation for the purpose of bone bank, which is signed along with agreement for surgery. They were previously anamnestically tested, clinically reviewed and adequate laboratory tests were conducted. Within multiorgan explantation heteroanamnestic data analysis was performed and all the necessary laboratory tests were taken under which we elected donors as favorable for donation of tissues and organs. It is custom in practice to conduct talks with the family regarding the donation of specific anatomical zones and agreeing and signing legalizes donation. After taking smears for bacteriological analysis grafts are packed and marked, and then saved in the freezer at -80°C. Method of deep freezing represents the most available and reliable technology for storage of bone grafts. In such conditions stored graft can be successfully used even after five years. Keywords bone bank, bone explantation, bone graft, deep freezing bone graft

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Osiguranje kvalitete i sigurnosti ko grafts stored by deep freezing meth Legović, Dalen; Šantić, Veljko; Gulan, Gordan; Jurdana, Hari; Tudor, Anton; Prpić, Tomislav & Šestan, Branko Koštana banka predstavlja složen sustav koji u svom radu ujedinjuje prikupljanje, pohranu i raspodjelu koštanih presadaka. Da bi se presadak adekvatno upotrijebio u koštano- -zglobnoj kirurgiji mora se mikrobiološki testirati i nadzirati, te, uz prateću dokumentaciju, obilježiti kada je spreman za upotrebu. Koštani presadci prikupljaju se ili od živućih donora koji daruju odstranjene dijelove glave bedrene kosti tijekom rutinskog zahvata ugradnje totalne endoproteze kuka, ili od kadavera, u sklopu multiorganske eksplantacije. Potencijalni živući davatelji daju svoj pristanak na donaciju za potrebe koštane banke, koja se potpisuje zajedno s pristankom na operacijski zahvat. Prethodno su anamnestički ispitani i klinički pregledani uz provedene odgovarajuće laboratorijski testove. U sklopu multiorganske eksplantacije izvršena je analiza heteroanamnestičkih podataka i poduzete su sve potrebne laboratorijske pretrage i testovi na osnovi kojih regrutiramo davatelja kao povoljnog za uzimanje tkiva i organa. U praksi je običaj da se obavi razgovor s obitelji o donaciji određene anatomske cjeline, te pristankom i potpisom legalizira davateljstvo. Presadci se, nakon uzimanje obriska za bakteriološku analizu, pakiraju i obilježavaju, te spremaju u zamrzivač na -80°C. Metoda dubokog smrzavanja predstavlja najdostupniju i pouzdanu tehniku pohranjivanja koštanih presadaka. U takvim uvjetima pohranjeni presadak može se uspješno upotrijebiti i nakon pet godina. alogeni presadci, duboko smrzavanje koštanih presadaka, eksplantacija presadaka, koštana banka

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