Malaysian Journal of Medical Sciences
School of Medical Sciences, Universiti Sains Malaysia
Vol. 17, No. 1, 2010, pp. 3-11
Bioline Code: mj10002
Full paper language: English
Document type: Research Article
Document available free of charge
Malaysian Journal of Medical Sciences, Vol. 17, No. 1, 2010, pp. 3-11
© Copyright 2010 - Malaysian Journal of Medical Science
Role of Pioglitazone with Metformin or Glimepiride on Oxidative Stress-Induced Nuclear Damage and Reproductive Toxicity in Diabetic Rats|
Rabbani, Syed Imam; Devi, Kshama & Khanam, Salma
Background: Oxidative stress due to improper control of blood glucose in chronic diabetes
plays a major role in the development of secondary complications including cancer and birth defect.
the aim of this study is to evaluate the protective effect of combination of pioglitazone with metformin
or glimepiride against the experimental type-2 diabetes induced nuclear damage and reproductive
toxicity in rats.
Methods: The combinations of Pioglitazone (Pio-1 mg/kg) with metformin (Met-50 mg/kg)
or glimepiride (Gmp-0.2 mg/kg) given orally daily for 4 weeks were tested against nicotinamide (nA-
230 mg/kg, ip)-streptozotocin (StZ-65 mg/kg, ip)-induced micronuclei (Mn) formation and sperm
abnormalities in male Wistar rats. the antioxidant status was evaluated by measuring the levels of
serum lipid peroxidation (LPO), catalase (CAt) and superoxide dismutase (SOD).
Results: The administration of Pio+Met significantly (P<0.01) reduced the number of
micronucleated erythrocytes, increased the polychromatic: normochromatic erythrocytes (P/n
ratio), reduced (P<0.001) sperm morphology defects and increased (P<0.05) the caudal sperm count
compared to the untreated diabetic condition. Furthermore, the Pio+Met combination enhanced the
antioxidant status in diabetic animals. However, Pio+Gmp did not attenuate the nuclear and sperm
defects or oxidative stress.
Conclusions: The observations suggest that Pio+Met combination reduced nuclear damage
and sperm abnormalities by enhancing the antioxidant status in the diabetic animals.
antidiabetics, combination therapy, micronuclei, sperm abnormalities, health sciences
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