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Malaysian Journal of Medical Sciences
School of Medical Sciences, Universiti Sains Malaysia
ISSN: 1394-195X
Vol. 18, No. 4, 2011, pp. 13-25
Bioline Code: mj11049
Full paper language: English
Document type: Research Article
Document available free of charge

Malaysian Journal of Medical Sciences, Vol. 18, No. 4, 2011, pp. 13-25

 en The In Vitro Antimicrobial Activities of Metabolites from Lactobacillus check for this species in other resources Strains on Candida check for this species in other resources Species Implicated in Candida Vaginitis
Ogunshe, Adenike A O; Omotoso, Mopelola A & Bello, Victoria B

Abstract

Background: Research from developing countries, such as Nigeria, on Lactobacillus check for this species in other resources species in the female urogenital tract and their role as a barrier to vaginal infection is limited. Therefore, the aim of this study was to assess the clinical biotherapeutic potential of indigenous Lactobacillus species.
Methods: Antimicrobial metabolites production were characterised using simple and easily reproducible qualitative and quantitative methods. The in vitro inhibitory effect of Lactobacillus antimicrobials on vulvovaginal candidiasis–associated Candida check for this species in other resources species was investigated using modified agar spot and agar well-diffusion methods.
Results: The maximum levels of lactic acid, hydrogen peroxide, and diacetyl from 20 vaginal Lactobacillus strains from diseased subjects were 1.46 mg/L, 1.36 mmol/L, and 1.72 mg/L respectively. From the 4 healthy subjects, the maximum level of lactic acid was 1.08 mg/L; hydrogen peroxide, 1.36 mmol/L; and diacetyl, 0.86 mg/L. The maximum productions of these substances occurred between 72 and 120 hours of incubation. The in vitro antagonistic activities of vaginal L. acidophilus check for this species in other resources , L. fermentum check for this species in other resources , L. brevis check for this species in other resources , L. plantarum check for this species in other resources , L. casei check for this species in other resources , L. delbrueckii check for this species in other resources , and L. jensenii check for this species in other resources from diseased subjects inhibited a maximum of 5.71% of the 35 Candida species tested, while vaginal L. acidophilus and L. plantarum from healthy subjects inhibited between 57.1% and 68.6% of Candida species in vitro.
Conclusion: Antimicrobial-producing lactobacilli can be considered as adjunct biotherapeutic candidates for the treatment of vulvovaginal candidiasis.

Keywords
antifungal agents, antimicrobial agents, Candida, contraceptives, Lactobacillus, vulvovaginal candidiasis

 
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