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Malaysian Journal of Medical Sciences
School of Medical Sciences, Universiti Sains Malaysia
ISSN: 1394-195X
Vol. 21, No. 3, 2014, pp. 47-54
Bioline Code: mj14033
Full paper language: English
Document type: Research Article
Document available free of charge

Malaysian Journal of Medical Sciences, Vol. 21, No. 3, 2014, pp. 47-54

 en Prevalence of Bacterial Contamination when using a Diversion Pouch during Blood Collection: A Single Center Study in Malaysia
JUMAAH, Norlaili; JOSHI, Sanmukh Ratilal & SANDAI, Doblin


  Background: The implementation of diversion pouches is to minimise the risk of bacterial contamination as the initial blood flow is prevented from entering primary bag collections as it is diverted into a pouch. This study was carried out to determine the prevalence of bacterial contamination in the diversion pouches used during blood collections in the Transfusion Department of Hospital Seberang Jaya, Penang, Malaysia.
  Methods: BD Bactec™ Fx instrument detection system was performed on 702 samples of 20 mL of diverting blood in diversion pouch. The inocullum volume was 10 mL for both aerobic and anaerobic bottles cultures and incubated for 5 days in the BD Bactec™ Fx instrument. Positive sample was flagged by BD Bactec™ Fx instrument and subculture to identify the species of organism.
  Results: The results showed that of 702 samples, 12 (1.7%) were contaminated. The bacterial species identified were coagulase negative Staphylococcus check for this species in other resources , Staphylococcus aureus check for this species in other resources and Gram positive Bacilli check for this species in other resources .
  Conclusion: The results strongly suggest that the usage of diversion pouch is of significant importance in reducing bacterial contamination during blood collection.

BD Bactec™ Fx; blood collection; blood transfusion; blood contamination; bacterial infections

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