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Malaysian Journal of Medical Sciences
School of Medical Sciences, Universiti Sains Malaysia
ISSN: 1394-195X
Vol. 22, No. 1, 2015, pp. 12-22
Bioline Code: mj15003
Full paper language: English
Document type: Research Article
Document available free of charge

Malaysian Journal of Medical Sciences, Vol. 22, No. 1, 2015, pp. 12-22

 en Quercus infectoria check for this species in other resources Gall Extract Enhanced the Proliferation and Activity of Human Fetal Osteoblast Cell Line (hFOB 1.19)
HAPIDIN, Hermizi; ROZELAN, Dalila; ABDULLAH, Hasmah; HANAFFI, Wan Nurhidayah Wan & SOELAIMAN, Ima Nirwana


Background: The present study investigated the effects of Quercus infectoria check for this species in other resources (QI) gall extract on the proliferation, alkaline phosphatase (ALP), osteocalcin, and the morphology of a human fetal osteoblast cell line (hFOB 1.19).
Methods: The cells were cultured in Dulbecco’s modified eagle medium F12 supplemented with a 10% fetal bovine serum, a 1% penicillin/streptomycin and were treated with QI at various concentrations (0.1 to 99.0 μg/mL) for 72 hours. The levels of ALP and osteocalcin were measured at day 1, 3, 7, 10, and 14 and were compared among the negative control, pamidronate and QI groups.
Results: The median effective concentration (EC50) of hFOB 1.19 treated with QI was 10.30 μg/mL. This concentration was more effective compared to the control drug, pamidronate (EC50 at 16.09 μg/mL). The ALP and osteocalcin levels of hFOB 1.19 treated with QI from day 7 and onwards were significantly increased in a time and concentration-dependent manner. Interestingly, from day 7 until day 14, the ALP and osteocalcin levels were highest in the cells treated with QI compared to the other two groups. The morphology of cells treated with QI was uniformly elongated, higher in number and over-confluent.
Conclusion: After treatment with QI, cell proliferation enhanced and ALP and osteocalcin levels increased.

Alkaline phosphatase (ALP); hFOB 1.19 cell line; MTT assay; osteocalcin; Quercus infectoria

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