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Malaysian Journal of Medical Sciences
School of Medical Sciences, Universiti Sains Malaysia
ISSN: 1394-195X
Vol. 22, No. 3, 2015, pp. 18-24
Bioline Code: mj15028
Full paper language: English
Document type: Research Article
Document available free of charge

Malaysian Journal of Medical Sciences, Vol. 22, No. 3, 2015, pp. 18-24

 en Mycobacterium tuberculosis check for this species in other resources DNA Increases Vitamin D Receptor mRNA Expression and the Production of Nitric Oxide and Cathelicidin in Human Monocytes
Siswanto, Siswanto; Zuhriyah, Lilik; Handono, Kusworini; Fitri, Loeki Enggar & Prawiro, Sumarno Reto

Abstract

Background: The innate immune response to tuberculosis infection may involve the increased production of nitric oxide and cathelicidin due to the up-regulated expression of the vitamin D receptor (VDR), though this proposed mechanism remains controversial. The aim of this study was to determine how the exposure of human monocytes to Mycobacterium tuberculosis check for this species in other resources (M. tuberculosis) DNA affects the production of nitric oxide and cathelicidin, as well as the expression of VDR.
Methods: This study was performed using monocytes obtained from healthy donors. After 24 h incubation, monocytes were stimulated with M. tuberculosis DNA for 18 h to determine the expression of VDR mRNA and the production of nitric oxide and cathelicidin versus non-stimulated cells (the control group).
Results: The expression of VDR mRNA was higher in the monocytes exposed to M. tuberculosis DNA compared to the control group (P = 0.020). Monocytes exposed to M. tuberculosis DNA also showed significantly increased production of nitric oxide and cathelicidin compared to the control group (P = 0.0001; P = 0.028).
Conclusion: The stimulation of human monocytes with M. tuberculosis DNA increases the expression of the VDR mRNA and the production of nitric oxide and cathelicidin.

Keywords
monocytes; Mycobacterium tuberculosis DNA; nitric oxide; cathelicidin; tuberculosis

 
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