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Malaysian Journal of Medical Sciences
School of Medical Sciences, Universiti Sains Malaysia
ISSN: 1394-195X
Vol. 23, No. 2, 2016, pp. 14-20
Bioline Code: mj16015
Full paper language: English
Document type: Research Article
Document available free of charge

Malaysian Journal of Medical Sciences, Vol. 23, No. 2, 2016, pp. 14-20

 en Antimicrobial Susceptibility of Klebsiella pneumoniae check for this species in other resources and Escherichia coli check for this species in other resources with Extended-Spectrum β-lactamase associated Genes in Hospital Tengku Ampuan Afzan, Kuantan, Pahang
Mahdi Yahya Mohsen, Saleh; Hamzah, Hairul Aini; Muhammad Imad Al-Deen, Mustafa & Baharudin, Roesnita

Abstract

Background: To assess antimicrobial susceptibility of extended-spectrum β-lactamase- (ESBL-) producing Klebsiella pneumoniae check for this species in other resources and Escherichia coli check for this species in other resources isolates from Hospital Tengku Ampuan Afzan (HTAA), as well as to identify ESBL genes.
Methods: Non-duplicate K. pneumoniae and E. coli isolates were recovered from various clinical samples. Isolates were screened for antimicrobial resistance by disc diffusion method. Isolates resistant to oxyimino-cephalosporins were subjected to phenotypic ESBL production. Detection of resistance genes was then performed using primers specific for ESBL genes (blaCTX-M, blaSHV and blaTEM).
Results: Piperacillin/tazobactam and carbapenems remained the active β-lactam antibiotic against K. pneumoniae and E. coli. ESBLs were detected among 35.5% (39/110) of K. pneumoniae and 18.8% (28/149) of E. coli isolates. CTX-M β-lactamase was detected in 90% of all ESBL-positive isolates, whereas blaSHV and blaTEM genes were found among 56% and 52% of them, respectively. Twenty-eight percent (28%) of the total ESBL-positive isolates harboured the three ESBL genes, while 50% carried two of the tested ESBL genes.
Conclusion: ESBLs encoded by at least one ESBL genes are frequently isolated among K. pneumoniae and E. coli in HTAA. The significant proportion rate of these resistant determinants is alarming, thus monitoring their transmission and dissemination is essential to control it at an early phase.

Keywords
beta-Lactamases; Escherichia coli; Klebsiella pneumoniae; antimicrobial resistance; hospital

 
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Alternative site location: http://www.medic.usm.my/publication/mjms/

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