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Malaysian Journal of Medical Sciences
School of Medical Sciences, Universiti Sains Malaysia
ISSN: 1394-195X
Vol. 23, No. 3, 2016, pp. 32-39
Bioline Code: mj16031
Full paper language: English
Document type: Research Article
Document available free of charge

Malaysian Journal of Medical Sciences, Vol. 23, No. 3, 2016, pp. 32-39

 en In Vitro Antifungal Activities against Moulds Isolated from Dermatological Specimens
Mohd Nizam, Tzar; Binting, Rabiatul Adawiyah AG.; Mohd Saari, Shafika; Kumar, Thivyananthini Vijaya; Muhammad, Marianayati; Satim, Hartini; Yusoff, Hamidah & Santhanam, Jacinta

Abstract

  Background: This study aimed to determine the minimum inhibitory concentrations (MICs) of various antifungal agents against moulds isolated from dermatological specimens.
  Methods: We identified 29 moulds from dermatological specimens between October 2012 and March 2013 by conventional methods. We performed antifungal susceptibility testing on six antifungal agents, amphotericin B, clotrimazole, itraconazole, ketoconazole, miconazole and terbinafine, according to the Clinical and Laboratory Standards Institute guidelines contained in the M38-A2 document.
  Results: Most antifungal agents were active against the dermatophytes, except for terbinafine against Trichophyton rubrum check for this species in other resources (geometric mean MIC, MICGM 3.17 μg/mL). The dematiaceous moulds were relatively susceptible to amphotericin B and azoles (MICGM 0.17-0.34 μg/mL), but not to terbinafine (MICGM 3.62 μg/mL). Septate hyaline moulds showed variable results between the relatively more susceptible Aspergillus check for this species in other resources (MICGM 0.25-4 μg/mL) and the more resistant Fusarium check for this species in other resources (MICGM 5.66-32 μg/mL). The zygomycetes were susceptible to amphotericin B (MICGM 0.5 μg/ mL) and clotrimazole (MICGM 0.08 μg/mL), but not to other azoles (MICGM 2.52-4 μg/mL).
  Conclusion: Amphotericin B and clotrimazole were the most effective antifungal agents against all moulds excepting Fusarium spp., while terbinafine was useful against dermatophytes (except T. rubrum) and Aspergillus spp. However, a larger study is required to draw more solid conclusions.

Keywords
antifungal; dermatology; mold; amphotericin B; azoles; terbinafine

 
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