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Malaysian Journal of Medical Sciences
School of Medical Sciences, Universiti Sains Malaysia
ISSN: 1394-195X
Vol. 23, No. 3, 2016, pp. 64-71
Bioline Code: mj16035
Full paper language: English
Document type: Research Article
Document available free of charge

Malaysian Journal of Medical Sciences, Vol. 23, No. 3, 2016, pp. 64-71

 en Differential Gene Expression of Heat Shock Protein 90 (Hsp90) of Candida albicans check for this species in other resources obtained from Malaysian and Iranian Patients
Khalili, Vajihe; Shokri, Hojjatollah; Akim, Abdah Md & Khosravi, Ali Reza


  Background: Candida albicans (C. albicans) has several virulence factors, in particular heat shock protein 90 (Hsp90), which is expressed by Hsp90 gene. The purposes of this study were to assess the expression of Hsp90 gene in clinical and control isolates of C. albicans obtained from different geographical regions (Malaysia and Iran), different temperatures (25ºC, 37ºC and 42ºC) and mice with candidiasis.
  Methods: C. albicans isolates were cultured onto sabouraud dextrose agar (SDA). The assessment of the expression of Hsp90 gene was performed using real time-polymerase chain reaction (RT-PCR).
   Results: The results showed a significant increase in the expression of C. albicans Hsp90 gene under high thermal shock (42ºC) when compared to other temperatures tested (P-value = 0.001). The mean differences in the expression of Hsp90 gene at 37ºC were 0.20 (95% confidence interval (CI) 0.13-0.29) between Malaysian and Iranian controls (P-value = 0.040) and 0.47 (95% CI 0.27-0.60) between Malaysian and Iranian patients (P-value = 0.040).
  Conclusion: The results demonstrated that the expression of C. albicans Hsp90 gene varied between Malaysian and Iranian subjects, representing the efficacy of geographical and thermal conditions on virulence gene expression.

Candidiasis; Candida albicans; gene expression; HSP90 Heat-Shock Proteins; Molecular Diagnostic Techniques

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