Cannabis-induced Moto-Cognitive Dysfunction in Wistar Rats: Ameliorative Efficacy of Nigella Sativa |
Imam, Aminu; Ajao, Moyosore Saliu; Amin, Abdulbasit; Abdulmajeed, Wahab Imam; Ibrahim, Abdulmumin; Olajide, Olayemi Joseph; Ajibola, Musa Iyiola; Alli-Oluwafuyi, Abdulmusawir & Balogun, Wasiu Gbolahan
Background: Cannabis is a widely used illicit drug with various threats of personality
syndrome, and Nigella sativa has been widely implicated as having therapeutic efficacy in many
neurological diseases. The present study investigates the ameliorative efficacy of Nigella sativa oil
(NSO) on cannabis-induced moto-cognitive defects.
Methods: Scopolamine (1 mg/kg i.p.) was given to induce dementia as a standard base
line for cannabis (20 mg/kg)-induced cognitive impairment, followed by an oral administration
of NSO (1 ml/kg) for 14 consecutive days. The Morris water maze (MWM) paradigm was used to
assess the memory index, the elevated plus maze was used for anxiety-like behaviour, and the
open field test was used for locomotor activities; thereafter, the rats were sacrificed and their
brains were removed for histopathologic studies.
Results: Cannabis-like Scopolamine caused memory impairment, delayed latency in
the MWM, and anxiety-like behaviour, coupled with alterations in the cerebello-hippocampal
neurons. The post-treatment of rats with NSO mitigated cannabis-induced cognitive dysfunction
as with scopolamine and impaired anxiety-like behaviour by increasing open arm entry, line
crossing, and histological changes.
Conclusions: The observed ameliorative effects of NSO make it a promising agent against
moto-cognitive dysfunction and cerebelo-hippocampal alterations induced by cannabis.
cannabis; toxicity; spatial memory; anxiety; brain