Malaysian Journal of Medical Sciences
School of Medical Sciences, Universiti Sains Malaysia
Vol. 24, No. 5, 2017, pp. 33-43
Bioline Code: mj17059
Full paper language: English
Document type: Research Article
Document available free of charge
Malaysian Journal of Medical Sciences, Vol. 24, No. 5, 2017, pp. 33-43
© Copyright 2017 - Penerbit Universiti Sains Malaysia
Detection of Respiratory Viruses from ARTI Patients by xTAG RVP Fast v2 Assay and Conventional Methods|
Kuan, Chee Sian; Yew, Su Mei; Hooi, Poh Sim; Lee, Lu Mei & Ng, Kee Peng
Introduction: Acute respiratory tract infections (ARTIs) are a major cause of morbidity
and mortality in paediatric patients. Therefore, early detection of the viral aetiologies of ARTIs
is essential for patient management and infection control. In this study, we evaluated the
performance of a new multiplex polymerase chain reaction (PCR) assay (xTAG Respiratory Viral
Panel [RVP] Fast v2) in the detection of respiratory viruses by comparing it with that of viral
culture and direct immunofluorescence (IF) staining.
Methods: Nasopharyngeal swab and aspirate samples were collected prospectively from
199 patients who presented with ARTIs at the University Malaya Medical Centre (UMMC) in
Kuala Lumpur, Malaysia during a 10-month period. The PCR assay was conducted in parallel with
conventional culture and direct IF staining methods.
Results: The positive rate of the xTAG RVP Fast v2 assay (78.4%) in detecting respiratory
viruses was higher than that of the viral isolation (7.5%) and direct IF (23.1%) methods. Using
the xTAG RVP Fast v2 assay, human enterovirus/human rhinovirus (HEV/HRV) was the most
frequently detected (46.2%). The xTAG RVP Fast v2 assay revealed mixed infection caused by two
or three respiratory viruses in 40 specimens, and these were undetected by the viral isolation and
direct IF methods.
Conclusion: The xTAG RVP Fast v2 assay was superior to conventional methods in the
identification of common respiratory viruses, with higher sensitivity and shorter turnaround
times for laboratory results.
respiratory tract infections; viruses; Multiplex PCR; diagnostic tests; routine
Alternative site location: http://www.medic.usm.my/publication/mjms/