Planimetric and Biomechanical Study of Local Effect of Pulegone on Full Thickness Wound Healing in Rat|
Cheraghali, Zahra; Mohammadi, Rahim & Jalilzadeh-Amin, Ghader
Background: Pulegone as principal component of essential oil, reported to have antibacterial,
antioxidant and anti-inflammatory properties. The present study was aimed to evaluate
wound healing activity of pulegone in a rat model.
Method: Forty rats were used for excisional and incisional wound healing models. For
each model twenty male white Wistar rats were randomly divided into five groups (n = 4) of
control (CG), Sham surgery, E1, E2 and E3. Wound size, hydroxyproline content of wound and
biomechanical testing were assessed.
Result: In E2 animals, the wound size was reduced earlier than in E1 and E2 groups
(P = 0.035). However, time had significant effect on wound contraction of all wounds.
Hydroxyproline contents in the groups CG, sham surgery, E1, E2 and E3 were found to be
51.25 ± 3.40, 58.41 ± 4.62, 68.59 ± 3.53, 86.32 ± 3.18, and 74.26 ± 4.73 mg g-1, respectively.
Hydroxyproline contents were increased significantly in E2 compared to E1 and E3 which implied
more collagen deposition compared to other experimental groups (P = 0.001). The biomechanical
indices, maximum stored energy, stiffness, ultimate strength and yield strength obtained for E2
group were significantly higher than those obtained for E1 and E2 groups (P = 0.002).
Conclusion: The pulegone showed a reproducible wound healing potential in rats.
cis-isopulegone; wound healing; planimertic; biomechanics